Cloned (Comment) | Organism |
---|---|
gene davT, recombinant expression of N-terminal His6-tagged enzyme in Corynebacterium glutamicum strain KCTC 1857, coexpression with glutarate semialdehyde dehydrogenase (davD, EC 1.2.1.20) from Pseudomonas putida, 4-aminobutyrate-2-oxoglutarate transaminase (gabT, EC 2.6.1.19) from Corynebacterium glutamicum, and NADP-dependent succinic semialdehyde dehydrogenase (gabD, EC 1.2.1.79) from Corynebacterium glutamicum, as well as N-terminal His6-tagged lysine 2-monooxygenase (davB, EC 1.13.12.2) from Pseudomonas putida | Pseudomonas putida |
gene dayB, recombinant expression in Corynebacterium glutamicum, co-expression with Pseudomonas putida dayA and dayB genes encoding encoding lysine 2-monooxygenase and delta-aminovaleramidase, respectively, and Corynebacterium glutamicum gabD gene encoding glutarate semialdehyde dehydrogenase | Corynebacterium glutamicum |
gene dayT, recombinant expression in Corynebacterium glutamicum, co-expression with Pseudomonas putida dayA, dayB, and dayD genes encoding lysine 2-monooxygenase, delta-aminovaleramidase, and glutarate semialdehyde dehydrogenase, respectively | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical by co-expression with Pseudomonas putida dayA, dayB, and dayD genes encoding encoding lysine 2-monooxygenase, delta-aminovaleramidase, and glutarate semialdehyde dehydrogenase, respectively, in Corynebacterium glutamicum. Method optimization and evaluation | Corynebacterium glutamicum |
additional information | metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical by co-expression with Pseudomonas putida dayA, dayB, and dayD genes encoding lysine 2-monooxygenase, delta-aminovaleramidase, and glutarate semialdehyde dehydrogenase, respectively, in Corynebacterium glutamicum. Method optimization and evaluation | Pseudomonas putida |
additional information | metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical, by co-expression of Pseudomonas putida davT, davB, and davD genes encoding lysine 2-monooxygenase, delta-aminovaleramidase, and glutarate semialdehyde dehydrogenase, respectively, in Corynebacterium glutamicum. Method optimization and evaluation. The glutaric acid biosynthesis pathway constructed in recombinant Corynebacterium glutamicum is engineered by examining strong synthetic promoters H30 and H36, Corynebacterium glutamicum codon-optimized davTDBA genes, and modification of davB gene with an N-terminal His6-tag to improve the production of glutaric acid. The use of N-terminal His6-tagged DavB is most suitable for the production of glutaric acid from glucose. Fed-batch fermentation on of the final engineered Corynebacterium glutamicum H30_GAHis strain, expressing davTDA genes along with davB fused with His6-tag at N-terminus can produce 24.5 g/l of glutaric acid with low accumulation of L-lysine (1.7 g/l), wherein 5-aminovaleric acid (5-AVA) ccumulation is not observed during fermentation. Metabolically engineered Corynebacterium glutamicum strain KCTC H30_GA-2 (engineered strain KCTC 1857) is able for catalysis of the biosynthesis of glutaric acid from glucose. Method optimization and evaluation, overview | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-aminopentanoate + 2-oxoglutarate | Pseudomonas putida | - |
5-oxopentanoate + L-glutamate | - |
? | |
5-aminopentanoate + 2-oxoglutarate | Pseudomonas putida DSM 6125 | - |
5-oxopentanoate + L-glutamate | - |
? | |
5-aminopentanoate + 2-oxoglutarate | Pseudomonas putida NCIMB 11950 | - |
5-oxopentanoate + L-glutamate | - |
? | |
5-aminopentanoate + 2-oxoglutarate | Pseudomonas putida ATCC 47054 | - |
5-oxopentanoate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | A0A0U4XQS6 | - |
- |
Pseudomonas putida | - |
- |
- |
Pseudomonas putida | Q88RB9 | - |
- |
Pseudomonas putida ATCC 47054 | Q88RB9 | - |
- |
Pseudomonas putida DSM 6125 | Q88RB9 | - |
- |
Pseudomonas putida NCIMB 11950 | Q88RB9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-aminopentanoate + 2-oxoglutarate | - |
Pseudomonas putida | 5-oxopentanoate + L-glutamate | - |
? | |
5-aminopentanoate + 2-oxoglutarate | - |
Pseudomonas putida DSM 6125 | 5-oxopentanoate + L-glutamate | - |
? | |
5-aminopentanoate + 2-oxoglutarate | - |
Pseudomonas putida NCIMB 11950 | 5-oxopentanoate + L-glutamate | - |
? | |
5-aminopentanoate + 2-oxoglutarate | - |
Pseudomonas putida ATCC 47054 | 5-oxopentanoate + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5-aminovalerate transaminase | - |
Pseudomonas putida |
5-aminovalerate transaminase | - |
Corynebacterium glutamicum |
davT | - |
Pseudomonas putida |
dayT | - |
Pseudomonas putida |
GabT | - |
Corynebacterium glutamicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
metabolism | in the L-lysine catabolism pathway, 5-aminovaleric acid (5-AVA) is furter converted into glutarate semialdehyde by 5-aminovalerate transaminase encoded by davT | Pseudomonas putida |